EDGE aims at communicating the diseases caused by herpesvirus, new approaches to treat such diseases, and cutting-edge research of EDGE on virus-host interactions and therapy to a broader audience. EDGE wants to broadly stimulate the public interest in research and innovation to inspire the next generation of researchers, developers and entrepreneurs. Information and teaching of the public involves participation in research nights, teaching and project programs, e.g. at local high schools. We further seek to disseminate our results to newspapers and other lay media after major goals have been achieved The aim of these communication activities is to bring updated knowledge on viruses and immunology to people working in closely related non-academic sectors, and also to inspire especially young people for a career in science and innovation.
International press release (in English) http://www.eurekalert.org/pub_releases/2016-04/au-jer041916.php
Local press release at Aarhus University, Denmark (in English) http://newsroom.au.dk/en/news/show/artikel/faelles-europaeisk-forskning-mod-herpesvirus/
One of the top-priority measures for the public outreach in EDGE is the communication of the consortiums’ work to a future generation of students. Hence, talks at schools, class invitations with seminars and guided lab-tours are a central part of the EDGE outreach activities.
As a start, Aarhus University organized an event with a local high school in November 2017, where the local ESRs (5, 6, 8 & 12) were involved (picture above). A high school class had its focus on the immune system in that semester and was therefore invited to visit the university to learn more about recent advances in research. And here, they got a particular insight into the work of the young researchers within the EDGE project. The ESRs presented their individual research projects in short PowerPoint talks. In discussion sessions, the high school students had the chance to visit a “typical” university lab and to get some first-hand impressions on the life of a young scientist in an academic environment, perhaps getting inspired to follow a similar career route in the future.
In a similar, yet more individual, fashion, ESRs 4 and 10 gave talks to school students to report on their research projects and personal experiences as PhD-students in an international collaboration. In September 2017, ESR10 presented her work to a German high school class, thereby addressing basic research in virology and informing the students about career-choices. ESR4 has given a guest talk and seminar in Molecular Microbiology for the graduate class of 2018 of Corso di Laurea in Tecniche di Laboratorio Biomedico in Cuneo, Piedmont/Italy on a topic of diagnostics of HCMV congenital infections, genotyping and sequencing techniques, presenting her own work as a member of the EDGE project and to generally demonstrate recent advances in Herpes research.
In the autumn break of 2018, ESRs 13 and 14 organized an outreach activity in their host lab at the Helmholtz Centre for Infection Research, Braunschweig/Germany. This activity was part of a larger school holidays arrangement of the “Haus der Wissenschaft in Braunschweig” (“House of science”, see https://www.hausderwissenschaft.org/) to excite children to work in science. The lab of the EDGE-ESRs hosted 19 students from 10-14 years old, who were introduced to the work of a biomedical lab and were allowed to even conduct their own experiments (picture below). The ESRs reported on their work at the institute in general and their experiences in participating in the EDGE programme in particular.
Encouraged by the practice of other lab groups and multinational research collaborations, the ESRs of EDGE launched the initiative to create an EDGE-Twitter account. This is meant to interconnect the students better, by having the instant option to post questions to peers and to exchange latest research advances, lab protocols and organizational information about conferences/workshops etc. However, the second intention, of course, is also the encouragement of other students/labs/consortia/laymen to follow the news of EDGE on Twitter:
Publications in peer-reviewed journals:
1) A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.
Praest P, de Buhr H, Wiertz EJHJ.
In: van Endert P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NYuture Virol. 2019 Apr 29. https://doi.org/10.2217/fvl-2018-0189
Galitska G, Biolatti M, Griffante G, Gugliesi F, Pasquero S, Dell'Oste V, Landolfo S.
Future Virol. 2019 Apr 29. https://doi.org/10.2217/fvl-2018-0189
Carter-Timofte ME, Paludan SR, Mogensen TH.
Trends Mol Med. 2018 Oct 24. (10):904-915. doi: 10.1016/j.molmed.2018.07.009. Epub 2018 Aug 13. Review.
4) The Viral Tegument Protein pp65 Impairs Transcriptional Upregulation of IL-1β by Human Cytomegalovirus through Inhibition of NF-kB Activity.
Biolatti M, Dell'Oste V, Scutera S, Gugliesi F, Griffante G, De Andrea M, Musso T, Landolfo S.
Viruses. 2018 Oct 16. doi: 10.3390/v10100567.
5) Biological relevance of Cytomegalovirus genetic variability in congenitally and postnatally infected children.
Galitska G, Biolatti M, De Andrea M, Leone A, Coscia A, Bertolotti L, Ala U, Bertino E, Dell'Oste V, Landolfo S.
J Clinic Virol. 2018 Oct 2. https://doi.org/10.1016/j.jcv.2018.09.019
de Buhr H, Lebbink RJ.
Curr Opin Immunol. 2018 Oct. 54:123-129. doi: 10.1016/j.coi.2018.06.002. Epub 2018 Jul 26. Review.
Carter-Timofte ME, Hansen AF, Mardahl M, Fribourg S, Rapaport F, Zhang SY, Casanova JL, Paludan SR, Christiansen M, Larsen CS, Mogensen TH.
Neurol Neuroimmunol Neuroinflamm. 2018 Sep 7. 5(6):e500. doi: 10.1212/NXI.0000000000000500.
De Buhr H, Lebbink RJ.
Blog for Desktop Genetics LtD. 2018
9) Evasion Strategy of Human Cytomegalovirus to Escape Interferon-β-Induced APOBEC3G Editing Activity.
Pautasso S, Galitska G, Dell'Oste V, Biolatti M, Cagliani R, Forni D, De Andrea M, Gariglio M, Sironi M, Landolfo S.
J Virol. 2018 Jul 25. pii: JVI.01224-18. doi: 10.1128/JVI.01224-18. [Epub ahead of print]
10) The influence of TAP1 and TAP2 gene polymorphisms on TAP function and its inhibition by viral immune evasion proteins.
Praest P, Luteijn RD, Brak-Boer IGJ, Lanfermeijer J, Hoelen H, Ijgosse L, Costa AI, Gorham RD Jr, Lebbink RJ, Wiertz EJHJ.
Mol Immunol. 2018 Jun 4;101:55-64. doi: 10.1016/j.molimm.2018.05.025. [Epub ahead of print]
11) Mutations in RNA Polymerase III genes and defective DNA sensing in adults with varicella-zoster virus CNS infection.
Carter-Timofte ME, Hansen AF, Christiansen M, Paludan SR, Mogensen TH.
Genes Immun. 2018 May 1. doi: 10.1038/s41435-018-0027-y. [Epub ahead of print]
12) New insights into the structure of the MHC class I peptide-loading complex and mechanisms of TAP inhibition by viral immune evasion proteins.
Praest P, Liaci AM, Förster F, Wiertz EJHJ.
Mol Immunol. 2018 Mar 29. pii: S0161-5890(18)30099-3. doi: 10.1016/j.molimm.2018.03.020. [Epub ahead of print]
13) Importin α1 is required for nuclear import of herpes simplex virus proteins and capsid assembly in fibroblasts and neurons.
Döhner K, Ramos-Nascimento A, Bialy D, Anderson F, Hickford-Martinez A, Rother F, Koithan T, Rudolph K, Buch A, Prank U, Binz A, Hügel S, Lebbink RJ, Hoeben RC, Hartmann E, Bader M, Bauerfeind R, Sodeik B.
PLoS Pathog. 2018 Jan 5;14(1):e1006823. doi: 10.1371/journal.ppat.1006823. eCollection 2018 Jan.
De Buhr H
Blog for Desktop Genetics LtD. 2018
Ramos-Nascimento A, Kellen B, Ferreira F, Alenquer M, Vale-Costa S, Raposo G, Delevoye C, Amorim MJ.
J Cell Sci. 2017 Dec 1;130(23):4038-4050. doi: 10.1242/jcs.210807. Epub 2017 Oct 23.
16) Cutting Edge: Genetic Association between IFI16 Single Nucleotide Polymorphisms and Resistance to Genital Herpes Correlates with IFI16 Expression Levels and HSV-2-Induced IFN-β Expression.
Eriksson K, Svensson A, Hait AS, Schlüter K, Tunbäck P, Nordström I, Padyukov L, Liljeqvist JÅ, Mogensen TH, Paludan SR.
J Immunol. 2017 Sep 11. pii: ji1700385. doi: 10.4049/jimmunol.1700385. [Epub ahead of print]
Ogunjimi B, Zhang SY, Sørensen KB, Skipper KA, Carter-Timofte M, Kerner G, Luecke S, Prabakaran T, Cai Y, Meester J, Bartholomeus E, Bolar NA, Vandeweyer G, Claes C, Sillis Y, Lorenzo L, Fiorenza RA, Boucherit S, Dielman C, Heynderickx S, Elias G, Kurotova A, Auwera AV, Verstraete L, Lagae L, Verhelst H, Jansen A, Ramet J, Suls A, Smits E, Ceulemans B, Van Laer L, Plat Wilson G, Kreth J, Picard C, Von Bernuth H, Fluss J, Chabrier S, Abel L, Mortier G, Fribourg S, Mikkelsen JG, Casanova JL, Paludan SR, Mogensen TH.
J Clin Invest. 2017 Sep 1;127(9):3543-3556. doi: 10.1172/JCI92280. Epub 2017 Aug 7.
18) PKC-δ isoform plays a crucial role in Tat-TLR4 signalling pathway to activate NF-κB and CXCL8 production.
Serrero M, Planès R, Bahraoui E.
Sci Rep. 2017 May 24;7(1):2384. doi: 10.1038/s41598-017-02468-8.