EDGE aims at communicating the diseases caused by herpesvirus, new approaches to treat such diseases, and cutting-edge research of EDGE on virus-host interactions and therapy to a broader audience. EDGE wants to broadly stimulate the public interest in research and innovation to inspire the next generation of researchers, developers and entrepreneurs. Information and teaching of the public involves participation in research nights, teaching and project programs, e.g. at local high schools. We further seek to disseminate our results to newspapers and other lay media after major goals have been achieved The aim of these communication activities is to bring updated knowledge on viruses and immunology to people working in closely related non-academic sectors, and also to inspire especially young people for a career in science and innovation.
International press release (in English) http://www.eurekalert.org/pub_releases/2016-04/au-jer041916.php
Local press release at Aarhus University, Denmark (in English) http://newsroom.au.dk/en/news/show/artikel/faelles-europaeisk-forskning-mod-herpesvirus/
One of the top-priority measures for the public outreach in EDGE is the communication of the consortiums’ work to a future generation of students. Hence, talks at schools, class invitations with seminars and guided lab-tours are a central part of the EDGE outreach activities.
As a start, Aarhus University organized an event with a local high school in November 2017, where the local ESRs (5, 6, 8 & 12) were involved (picture above). A high school class had its focus on the immune system in that semester and was therefore invited to visit the university to learn more about recent advances in research. And here, they got a particular insight into the work of the young researchers within the EDGE project. The ESRs presented their individual research projects in short PowerPoint talks. In discussion sessions, the high school students had the chance to visit a “typical” university lab and to get some first-hand impressions on the life of a young scientist in an academic environment, perhaps getting inspired to follow a similar career route in the future.
In a similar, yet more individual, fashion, ESRs 4 and 10 gave talks to school students to report on their research projects and personal experiences as PhD-students in an international collaboration. In September 2017, ESR10 presented her work to a German high school class, thereby addressing basic research in virology and informing the students about career-choices. ESR4 has given a guest talk and seminar in Molecular Microbiology for the graduate class of 2018 of Corso di Laurea in Tecniche di Laboratorio Biomedico in Cuneo, Piedmont/Italy on a topic of diagnostics of HCMV congenital infections, genotyping and sequencing techniques, presenting her own work as a member of the EDGE project and to generally demonstrate recent advances in Herpes research.
In the autumn break of 2018, ESRs 13 and 14 organized an outreach activity in their host lab at the Helmholtz Centre for Infection Research, Braunschweig/Germany. This activity was part of a larger school holidays arrangement of the “Haus der Wissenschaft in Braunschweig” (“House of science”, see https://www.hausderwissenschaft.org/) to excite children to work in science. The lab of the EDGE-ESRs hosted 19 students from 10-14 years old, who were introduced to the work of a biomedical lab and were allowed to even conduct their own experiments (picture below). The ESRs reported on their work at the institute in general and their experiences in participating in the EDGE programme in particular.
Following the success of this event, HZI participated in a similar action in March 2019 again. ESRs 13 and 14 hosted school students within an event called “Zukunftstag” (“Future day”), an initiative to present future career options to school students. The ESRs of EDGE demonstrated the research practice of a biomedical scientist in an according laboratory course, which they self-organized.
Encouraged by the practice of other lab groups and multinational research collaborations, the ESRs of EDGE launched the initiative to create an EDGE-Twitter account. This is meant to interconnect the students better, by having the instant option to post questions to peers and to exchange latest research advances, lab protocols and organizational information about conferences/workshops etc. However, the second intention, of course, is also the encouragement of other students/labs/consortia/laymen to follow the news of EDGE on Twitter:
ESR 7 participated in a public science event called “Science Me!” that took place in Geneva, Switzerland, in July 2018 during the traditional “Nuit de la Science” (“Science night”). This event is a recurring European Science Show Competition, an innovative instrument offering to the general audience the possibility to evaluate -at the measuring rod of their own sensitivity and understanding- short presentations in all fields of science or technology given in a playful and interactive manner (website: http://scienceme.unige.ch/). ESR7, Elisa Rossetti, together with Valeria Cagno and Giovanni Porcellana, thereby won the Jury Special Prize for their presentation: “Virus vs Immune system – The Battle!”
The ESRs 4 and 9 organized their own Public Science event entitled: "A pathogen lurking inside: come learn about your hostmate! Herpesvirus, a true story". In June 2019, they presented the MSCA and our EDGE-programme to the interested public and university students alike (see advertisement: http://www.viplab.unito.it/it/node/7). Apart from generally talking about the structure, the goals and purposes, the students also presented scientific results of their Herpes virus research, tailored to a lay audience.
Publications in peer-reviewed journals:
1) Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans.
Hait AS, Olagnier D, Sancho-Shimizu V, Skipper KA, Helleberg M, Larsen SM, Bodda C, Moldovan LI, Ren F, Brinck Andersen NS, Thomsen MM, Freytag MR, Darmalinggam S, Parkes I, Kadekar DD, Rahbek SH, van der Horst D, Kristensen LS, Eriksson K, Kjems J, Mostowy S, Christiansen M, Mikkelsen JG, Brandt CT, Paludan SR, Mogensen TH.
Sci Immunol. 2020 Dec 11. 5(54):eabc2691. doi: 10.1126/sciimmunol.abc2691.
2) A Broad-Spectrum Antiviral Peptide Blocks Infection of Viruses by Binding to Phosphatidylserine in the Viral Envelope.
Luteijn RD, Praest P, Thiele F, Sadasivam SM, Singethan K, Drijfhout JW, Bach C, de Boer SM, Lebbink RJ, Tao S, Helfer M, Bach NC, Protzer U, Costa AI, Killian JA, Drexler I, Wiertz EJHJ.
Cells. 2020 Aug 29. 9(9):1989. doi: 10.3390/cells9091989.
Zhang BC, Nandakumar R, Reinert LS, Huang J, Laustsen A, Gao ZL, Sun CL, Jensen SB, Troldborg A, Assil S, Berthelsen MF, Scavenius C, Zhang Y, Windross SJ, Olagnier D, Prabakaran T, Bodda C, Narita R, Cai Y, Zhang CG, Stenmark H, Doucet CM, Noda T, Guo Z, Goldbach-Mansky R, Hartmann R, Chen ZJ, Enghild JJ, Bak RO, Thomsen MK, Paludan SR.
Nat Immunol. 2020 Aug 21. 1(8):868-879. doi: 10.1038/s41590-020-0730-5.
Hait AS, Thomsen MM, Larsen SM, Helleberg M, Mardahl M, Barfod TS, Christiansen M, Brandt C, Mogensen TH.
J Infect Dis. 2020 Sep 18. jiaa589. doi: 10.1093/infdis/jiaa589.
5) The Cytomegalovirus Tegument Protein UL35 Antagonizes Pattern Recognition Receptor-Mediated Type I IFN Transcription.
Fabits M, Gonçalves Magalhães V, Chan B, Girault V, Elbasani E, Rossetti E, Saeland E, Messerle M, Pichlmair A, Lisnić VJ, Brinkmann MM.
Microorganisms. 2020 May 26. 8(6):790. doi: 10.3390/microorganisms8060790.
Coscia A, Leone A, Rubino C, Galitska G, Biolatti M, Bertino E, Peila C, Cresi F.
Microorganisms. 2020 May 25. 8(5):786. doi: 10.3390/microorganisms8050786.
Gugliesi F, Coscia A, Griffante G, Galitska G, Pasquero S, Albano C, Biolatti M.
Microorganisms. 2020 May 8. 8(5):685. doi: 10.3390/microorganisms8050685.
Dell'Oste V, Biolatti M, Galitska G, Griffante G, Gugliesi F, Pasquero S, Zingoni A, Cerboni C, De Andrea M.
Front Microbiol. 2020 Apr 15. 11:661. doi: 10.3389/fmicb.2020.00661.
Hertzog J, Rigby RE, Roll S, Cursi C, Chauveau L, Davenne T, Rehwinkel J.
BioRxiv. 2020 Feb 11. doi: https://doi.org/10.1101/2020.02.11.943415.
10) One Step Ahead: Herpesviruses Light the Way to Understanding Interferon-Stimulated Genes (ISGs).
Gonzalez-Perez AC, Stempel M, Chan B, Brinkmann MM.
Front Microbiol. 2020 Feb 7. 11:124. doi: 10.3389/fmicb.2020.00124.
11) Human SNORA31 variations impair cortical neuron-intrinsic immunity to HSV-1 and underlie herpes simplex encephalitis.
Lafaille FG, Harschnitz O, Lee YS, Zhang P, Hasek ML, Kerner G, Itan Y, Ewaleifoh O, Rapaport F, Carlile TM, Carter-Timofte ME, Paquet D, Dobbs K, Zimmer B, Gao D, Rojas-Duran MF, Kwart D, Rattina V, Ciancanelli MJ, McAlpine JL, Lorenzo L, Boucherit S, Rozenberg F, Halwani R, Henry B, Amenzoui N, Alsum Z, Marques L, Church JA, Al-Muhsen S, Tardieu M, Bousfiha AA, Paludan SR, Mogensen TH, Quintana-Murci L, Tessier-Lavigne M, Smith GA, Notarangelo LD, Studer L, Gilbert W, Abel L, Casanova JL, Zhang SY.
Nat Med. 2019 Dec 25. 25(12):1873-1884. doi: 10.1038/s41591-019-0672-3.
12) Fluorescent TAP as a Platform for Virus-Induced Degradation of the Antigenic Peptide Transporter.
Wąchalska M, Graul M, Praest P, Luteijn RD, Babnis AW, Wiertz EJHJ, Bieńkowska-Szewczyk K, Lipińska AD.
Cells. 2019 Dec 7. 8(12):1590. doi: 10.3390/cells8121590.
13) HSV-1 triggers paracrine fibroblast growth factor response from cortical brain cells via immediate-early protein ICP0.
Hensel N, Raker V, Förthmann B, Detering NT, Kubinski S, Buch A, Katzilieris-Petras G, Spanier J, Gudi V, Wagenknecht S, Kopfnagel V, Werfel TA, Stangel M, Beineke A, Kalinke U, Paludan SR, Sodeik B, Claus P.
J Neuroinflammation. 2019 Dec 2. 248. doi: 10.1186/s12974-019-1647-5.
14) A Conserved Mechanism of APOBEC3 Relocalization by Herpesviral Ribonucleotide Reductase Large Subunits.
Cheng AZ, Nóbrega de Moraes S, Attarian C, Yockteng-Melgar J, Jarvis MC, Biolatti M, Galitska G, Dell'Oste V, Frappier L, Bierle CJ, Rice SA, Harris RS.
J Virol. 2019 Sep 18. pii: JVI.01539-19. doi: 10.1128/JVI.01539-19.
Nandakumar R, Windross SJ, Paludan SR.
Methods Enzymol. 2019 Jul 23. 625:1-11. doi: 10.1016/bs.mie.2019.07.007.
Mardahl M, Jørgensen SE, Schneider A, Raaschou-Jensen K, Holm M, Veirum J, Kristensen TK, Johansen IS, Christiansen M, Assing K, Mogensen TH.
Br J Haematol. 2019 May 20. 186(3):471-476. doi: 10.1111/bjh.15947.
17) A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.
Praest P, de Buhr H, Wiertz EJHJ.
In: van Endert P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NYuture Virol. 2019 Apr 29. https://doi.org/10.2217/fvl-2018-0189
Galitska G, Biolatti M, Griffante G, Gugliesi F, Pasquero S, Dell'Oste V, Landolfo S.
Future Virol. 2019 Apr 29. https://doi.org/10.2217/fvl-2018-0189
Hooykaas MJG, Soppe JA, De Buhr HM, Kruse E, Wiertz EJHJ, Lebbink RJ.
RNA Biol. 2018 Nov 7. 15(11):1410-1419. doi:10.1080/15476286.2018.1537746018.
Carter-Timofte ME, Paludan SR, Mogensen TH.
Trends Mol Med. 2018 Oct 24. (10):904-915. doi: 10.1016/j.molmed.2018.07.009. Epub 2018 Aug 13. Review.
21) The Viral Tegument Protein pp65 Impairs Transcriptional Upregulation of IL-1β by Human Cytomegalovirus through Inhibition of NF-kB Activity.
Biolatti M, Dell'Oste V, Scutera S, Gugliesi F, Griffante G, De Andrea M, Musso T, Landolfo S.
Viruses. 2018 Oct 16. doi: 10.3390/v10100567.
22) Biological relevance of Cytomegalovirus genetic variability in congenitally and postnatally infected children.
Galitska G, Biolatti M, De Andrea M, Leone A, Coscia A, Bertolotti L, Ala U, Bertino E, Dell'Oste V, Landolfo S.
J Clinic Virol. 2018 Oct 2. https://doi.org/10.1016/j.jcv.2018.09.019
de Buhr H, Lebbink RJ.
Curr Opin Immunol. 2018 Oct. 54:123-129. doi: 10.1016/j.coi.2018.06.002. Epub 2018 Jul 26. Review.
Carter-Timofte ME, Hansen AF, Mardahl M, Fribourg S, Rapaport F, Zhang SY, Casanova JL, Paludan SR, Christiansen M, Larsen CS, Mogensen TH.
Neurol Neuroimmunol Neuroinflamm. 2018 Sep 7. 5(6):e500. doi: 10.1212/NXI.0000000000000500.
De Buhr H, Lebbink RJ.
Blog for Desktop Genetics LtD. 2018
Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J.
Eur J Immunol. 2018 Jul. 48(7):1120-1136. doi: 10.1002/eji.201847483.
27) Evasion Strategy of Human Cytomegalovirus to Escape Interferon-β-Induced APOBEC3G Editing Activity.
Pautasso S, Galitska G, Dell'Oste V, Biolatti M, Cagliani R, Forni D, De Andrea M, Gariglio M, Sironi M, Landolfo S.
J Virol. 2018 Jul 25. pii: JVI.01224-18. doi: 10.1128/JVI.01224-18. [Epub ahead of print]
28) The influence of TAP1 and TAP2 gene polymorphisms on TAP function and its inhibition by viral immune evasion proteins.
Praest P, Luteijn RD, Brak-Boer IGJ, Lanfermeijer J, Hoelen H, Ijgosse L, Costa AI, Gorham RD Jr, Lebbink RJ, Wiertz EJHJ.
Mol Immunol. 2018 Jun 4;101:55-64. doi: 10.1016/j.molimm.2018.05.025. [Epub ahead of print]
29) Mutations in RNA Polymerase III genes and defective DNA sensing in adults with varicella-zoster virus CNS infection.
Carter-Timofte ME, Hansen AF, Christiansen M, Paludan SR, Mogensen TH.
Genes Immun. 2018 May 1. doi: 10.1038/s41435-018-0027-y. [Epub ahead of print]
30) New insights into the structure of the MHC class I peptide-loading complex and mechanisms of TAP inhibition by viral immune evasion proteins.
Praest P, Liaci AM, Förster F, Wiertz EJHJ.
Mol Immunol. 2018 Mar 29. pii: S0161-5890(18)30099-3. doi: 10.1016/j.molimm.2018.03.020. [Epub ahead of print]
31) Importin α1 is required for nuclear import of herpes simplex virus proteins and capsid assembly in fibroblasts and neurons.
Döhner K, Ramos-Nascimento A, Bialy D, Anderson F, Hickford-Martinez A, Rother F, Koithan T, Rudolph K, Buch A, Prank U, Binz A, Hügel S, Lebbink RJ, Hoeben RC, Hartmann E, Bader M, Bauerfeind R, Sodeik B.
PLoS Pathog. 2018 Jan 5;14(1):e1006823. doi: 10.1371/journal.ppat.1006823. eCollection 2018 Jan.
De Buhr H
Blog for Desktop Genetics LtD. 2018
Ramos-Nascimento A, Kellen B, Ferreira F, Alenquer M, Vale-Costa S, Raposo G, Delevoye C, Amorim MJ.
J Cell Sci. 2017 Dec 1;130(23):4038-4050. doi: 10.1242/jcs.210807. Epub 2017 Oct 23.
34) Cutting Edge: Genetic Association between IFI16 Single Nucleotide Polymorphisms and Resistance to Genital Herpes Correlates with IFI16 Expression Levels and HSV-2-Induced IFN-β Expression.
Eriksson K, Svensson A, Hait AS, Schlüter K, Tunbäck P, Nordström I, Padyukov L, Liljeqvist JÅ, Mogensen TH, Paludan SR.
J Immunol. 2017 Sep 11. pii: ji1700385. doi: 10.4049/jimmunol.1700385. [Epub ahead of print]
Ogunjimi B, Zhang SY, Sørensen KB, Skipper KA, Carter-Timofte M, Kerner G, Luecke S, Prabakaran T, Cai Y, Meester J, Bartholomeus E, Bolar NA, Vandeweyer G, Claes C, Sillis Y, Lorenzo L, Fiorenza RA, Boucherit S, Dielman C, Heynderickx S, Elias G, Kurotova A, Auwera AV, Verstraete L, Lagae L, Verhelst H, Jansen A, Ramet J, Suls A, Smits E, Ceulemans B, Van Laer L, Plat Wilson G, Kreth J, Picard C, Von Bernuth H, Fluss J, Chabrier S, Abel L, Mortier G, Fribourg S, Mikkelsen JG, Casanova JL, Paludan SR, Mogensen TH.
J Clin Invest. 2017 Sep 1;127(9):3543-3556. doi: 10.1172/JCI92280. Epub 2017 Aug 7.
36) PKC-δ isoform plays a crucial role in Tat-TLR4 signalling pathway to activate NF-κB and CXCL8 production.
Serrero M, Planès R, Bahraoui E.
Sci Rep. 2017 May 24;7(1):2384. doi: 10.1038/s41598-017-02468-8.