ESR10 - Identification and characterization of new cytosolic HSV-1 restriction factors

Medizinische Hochschule Hannover, Germany; Supervisor: Beate Sodeik

Objectives

  • To identify novel cytosolic proteases which restrict incoming capsids and genomes of alphaherpesviruses
  • To characterize the molecular mechanisms how these proteases contribute to intrinsic host resistance against HSV-1, HSV-2 and VZV
  • To characterize the role of such proteases in host defense of primary macrophages, other immune cells and neurons

Expected achievements

  • Extensive characterization of infection (virus binding, internalization, microtubule transport, nuclear targeting, genome uncoating) after infection in the presence or absence of specific host proteases with a focus on HSV-1 and comparison to HSV-1 and VZV
  • This project will identify host proteases of macrophages or dendritic cells that contribute to targeting herpesvirus capsids to autophagosomes or cytosolic proteases for degradation and inactivation, and correlate this information to proteases that contribute to efficient nuclear targeting of incoming capsids in epithelial cells and neurons

Planned Secondments

  • Jena Biosciences GmbH, Germany; Label single HSV-1 genomes by high-resolution fluorescence microscopy
  • University Medical Centre Utrecht, The Netherlands; CRISPR/Cas technology
  • Aarhus University Hospital, Denmark; Clinical Aspects of herpesvirus infections

Requirements

  • Excellent background in cell biology; an expertise in virology would be an asset
  • Experience with state-of-the-art fluorescence microscopy. Knowledge on life cell imaging and advanced digital image processing would be an asset
  • Ability to manage complex experimental setups and large data sets

Appointment details

Based on a full-time appointment, the duration of the appointment as a Marie Curie ITN fellow will be for a maximum period of 3 years. The PhD student will become member of the Hannover Biomedical Research School in the Program of Infection Biology (ZIB) at Hannover Medical School, and is expected to present her/his work at the annually ZIB Symposium and to follow the mandatory training program.

 


 

Contact details

Beate Sodeik, Professor

Institute of Virology

Hannover Medical School

Hannover, Germany

This email address is being protected from spambots. You need JavaScript enabled to view it.

www.mh-hannover.de/ag-sodeik.html